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cleaved il18  (Proteintech)


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    Structured Review

    Proteintech cleaved il18
    Cleaved Il18, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+18/IL-18+Antibody/pm42805067-76-38-40
    Average 96 stars, based on 520 article reviews
    cleaved il18 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Statins targeting mevalonate-geranylgeranyl diphosphate pathway inhibit proliferation in NK/T lymphoma by promoting pyroptosis.
    Article Snippet: After electrophoretic separation via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the proteins were electroblotted onto nitrocellulose membranes (66485, PALL, USA) using a semi-dry transfer system. .. The membranes were then incubated with primary antibodies against pro-caspase-1 (1:1000, ab179515, Abcam, England), cleaved caspase-1 (1:1000, 4199, CST, USA), GSDMD (1:2000, 20770-1-AP, Proteintech, Wuhan, China), cleaved N-terminal GSDMD (1:1000, ab215203, Abcam), IL-18 (1:5000, 10663-1-AP, Proteintech), IL-1β (1:1,000, 26048-1-AP, Proteintech), GSDME (1:2000, 13075-1-AP, Proteintech) and β-actin (1:5000, 66009-1-lg, Proteintech) overnight at 4 ◦C. .. Then the membranes were cultured with secondary antibodies (1:5000, SA00001-1 and SA00001-2, Proteintech) for 1 hour at room temperature.

    Article Title: Hungatella hathewayi : A Tumor-Derived Bacterium Enriched in Colorectal Cancer Tissues and a Potential Diagnostic Biomarker.
    Article Snippet: .. The membranes were incubated overnight at 4 ◦C with primary antibodies targeting NLRP3 (Cat No. 30109-1-AP, ProteinTech Group, Inc., Rosemont, IL, USA), IL- https://doi.org/10.3390/microorganisms14030707 1β (516288, ZEN-BIOSCIENCE Co., Ltd., Chengdu, China), ASC (Cat No. 10500-1-AP, ProteinTech Group, Inc.), Caspase-1 (Cat No. 22915-1-AP, ProteinTech Group, Inc.), IL-18 (Cat No. 10663-1-AP, ProteinTech Group, Inc.), HIF-1α (Cat. PB9253, Boster Biological Technology Co., Ltd., Wuhan, China), CDH1 (Cat. PTM-6222, PTM BioLab Inc., Hangzhou, China), Vimentin (Cat. PTM-5376, PTM BioLab Inc.), Snail1 (Cat. 3879, Cell Signaling Technology, Inc., Danvers, MA, USA), and β-actin (Cat. PTM-5455, PTM BioLab Inc.), followed by a 1 h incubation with secondary antibodies (Cat No. SA00001-2, ProteinTech Group, Inc.) at room temperature. .. After washing, the membranes were visualized using Ultra High Sensitivity ECL Kit (GLPBIO, Montclair, CA, USA; cat. no. GK10008).

    Article Title: Hungatella hathewayi : A Tumor-Derived Bacterium Enriched in Colorectal Cancer Tissues and a Potential Diagnostic Biomarker
    Article Snippet: .. The membranes were incubated overnight at 4 °C with primary antibodies targeting NLRP3 (Cat No. 30109-1-AP, ProteinTech Group, Inc., Rosemont, IL, USA), IL-1β (516288, ZEN-BIOSCIENCE Co., Ltd., Chengdu, China), ASC (Cat No. 10500-1-AP, ProteinTech Group, Inc.), Caspase-1 (Cat No. 22915-1-AP, ProteinTech Group, Inc.), IL-18 (Cat No. 10663-1-AP, ProteinTech Group, Inc.), HIF-1α (Cat. PB9253, Boster Biological Technology Co., Ltd., Wuhan, China), CDH1 (Cat. PTM-6222, PTM BioLab Inc., Hangzhou, China), Vimentin (Cat. PTM-5376, PTM BioLab Inc.), Snail1 (Cat. 3879, Cell Signaling Technology, Inc., Danvers, MA, USA), and β-actin (Cat. PTM-5455, PTM BioLab Inc.), followed by a 1 h incubation with secondary antibodies (Cat No. SA00001-2, ProteinTech Group, Inc.) at room temperature. .. After washing, the membranes were visualized using Ultra High Sensitivity ECL Kit (GLPBIO, Montclair, CA, USA; cat. no. GK10008).

    Article Title: ADSC-Conditioned Medium Mitigates LPS-Induced Acute Lung Injury by Inhibiting Alveolar Macrophage Pyroptosis.
    Article Snippet: The separated proteins were then transferred onto nitrocellulose membranes with pore sizes of 0.22 μm or 0.45 μm (Biosharp, Hefei, China) at 4 ◦C. .. The membranes were blocked with 5% blocking-grade skim milk (BioFroxx, Guangzhou, China) for 1 h, followed by an overnight incubation at 4 ◦C on a shaker with primary antibodies: TLR4 (1:1000, Abcam, ab22048, Cambridge, MA, USA), MyD88 (1:1000, Wanleibio, WL02494, Shenyang, China), IKKα/β (1:1000, Wanleibio, WL01900), NF-κB (1:2000, Affinity, BF8005, Liyang, China), NLRP3 (1:800, Affinity, BF8029), Pro Caspase-1 (1:3000, Immunoway, YM8437, San Jose, CA, USA), Caspase-1 (1:1500, Wanleibio, WL03450), Pro GSDMD, GSDMD (1:800, Affinity, AF4012), Pro IL-1β, IL-1β (1:3000, Immunoway, YM8498) and IL-18 (1:2000, Proteintech, 60070-1-Ig, Wuhan, China). ..

    TUNEL Assay:

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy
    Article Snippet: .. Streptozotocin (STZ) was purchased from Sigma-Aldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy.
    Article Snippet: .. Streptozotocin (STZ) was purchased from SigmaAldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy
    Article Snippet: .. Streptozotocin (STZ) was purchased from Sigma-Aldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy.
    Article Snippet: .. Streptozotocin (STZ) was purchased from SigmaAldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Modification:

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy
    Article Snippet: .. Streptozotocin (STZ) was purchased from Sigma-Aldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy.
    Article Snippet: .. Streptozotocin (STZ) was purchased from SigmaAldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    CCK-8 Assay:

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy
    Article Snippet: .. Streptozotocin (STZ) was purchased from Sigma-Aldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Article Title: Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy.
    Article Snippet: .. Streptozotocin (STZ) was purchased from SigmaAldrich (St. Louis, MO, USA); brilliant blue G (BBG) from Technology Co., Ltd. (Beijing, China); a Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit from Hoffmann-La Roche (Basel, Switzerland); enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 from Elabscience (Wuhan, China); fetal bovine serum (FBS) and HG Dulbecco’s modified Eagle’s medium (DMEM) from Gibco (USA); a cell counting kit-8 (CCK-8) from Lanjieke Technology Co., LTD (Beijing, China); a lactate dehydrogenase (LDH) kit from Jianglai Biotechnology Co., LTD (Shanghai, China); antibodies against polyclonal GSDMD, GSDMD-N, Caspase 1, CASPASE 1 P10, and NLRP3 from Invitrogen (Carlsbad, CA, USA); antibodies against ASC, IL-18, and IL-1β from Proteintech (Wuhan, China); antibodies against P2X7R from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and BzATP triethylammonium salt and A438079 hydrochloride were purchased from MedChemExpress (USA); Astragaloside IV from Wansheng Pharmaceutical Co., Ltd (Zhejiang, China) and Puerarin from Must Bio-Technology Co., Ltd. (Chengdu, China). ..

    Blocking Assay:

    Article Title: ADSC-Conditioned Medium Mitigates LPS-Induced Acute Lung Injury by Inhibiting Alveolar Macrophage Pyroptosis.
    Article Snippet: The separated proteins were then transferred onto nitrocellulose membranes with pore sizes of 0.22 μm or 0.45 μm (Biosharp, Hefei, China) at 4 ◦C. .. The membranes were blocked with 5% blocking-grade skim milk (BioFroxx, Guangzhou, China) for 1 h, followed by an overnight incubation at 4 ◦C on a shaker with primary antibodies: TLR4 (1:1000, Abcam, ab22048, Cambridge, MA, USA), MyD88 (1:1000, Wanleibio, WL02494, Shenyang, China), IKKα/β (1:1000, Wanleibio, WL01900), NF-κB (1:2000, Affinity, BF8005, Liyang, China), NLRP3 (1:800, Affinity, BF8029), Pro Caspase-1 (1:3000, Immunoway, YM8437, San Jose, CA, USA), Caspase-1 (1:1500, Wanleibio, WL03450), Pro GSDMD, GSDMD (1:800, Affinity, AF4012), Pro IL-1β, IL-1β (1:3000, Immunoway, YM8498) and IL-18 (1:2000, Proteintech, 60070-1-Ig, Wuhan, China). ..



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    a Gene set enrichment analysis of Vγ9Vδ2 γδ T cells compared with non-Vγ9Vδ2 γδ T cells using published MAIT cell signatures ( GSE200173 ). NES, normalized enrichment score. b Proportion of IFN-γ + cells among non-Vδ2 and Vδ2 γδ T cells after IL-12 <t>and</t> <t>IL-18</t> stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). c Violin plots showing ADT expression levels of CD26 and CD161 across Vγ9Vδ2 γδ T cell clusters (C1 and C2/C3). Horizontal lines indicate the median expression level. d Representative flow cytometry plots of Vδ2 γδ T-cell clusters, categorized into CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells. e Representative flow cytometry histograms and cumulative data presenting the PLZF expression in the Vδ2 γδ T-cell population ( n = 23 biologically independent donors). Violin plots showing expressions of MAIT gene set module score ( f ) and KLRB1 ( g ) in Vγ9Vδ2 clusters (C1–C3). Horizontal lines indicate the median expression level. h Proportions of IFN-γ + and TNF + cells among CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells after IL-12 and IL-18 stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). i Expression of IL-18Rα (CD218a) in the Vδ2 γδ T-cell population ( n = 12 biologically independent donors). j Representative flow cytometry plots and proportion of IL-17A + cells among CD26 + CD161 + (C1), non-CD26 + CD161 + (C2/C3), and MAIT cells as a positive control from peripheral blood (squares) and liver sinusoidal (circles) mononuclear cells after PMA/ionomycin stimulation (10 ng/mL; 500 ng/mL, 6 h) ( n = 8 biologically independent donors). Data are presented as mean ± s.d. Statistics: Gene set enrichment analysis with false discovery rate correction ( a ); two-sided Wilcoxon signed-rank tests ( b , e , h , i , j ); Source data are provided as a file.
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    a Gene set enrichment analysis of Vγ9Vδ2 γδ T cells compared with non-Vγ9Vδ2 γδ T cells using published MAIT cell signatures ( GSE200173 ). NES, normalized enrichment score. b Proportion of IFN-γ + cells among non-Vδ2 and Vδ2 γδ T cells after IL-12 and IL-18 stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). c Violin plots showing ADT expression levels of CD26 and CD161 across Vγ9Vδ2 γδ T cell clusters (C1 and C2/C3). Horizontal lines indicate the median expression level. d Representative flow cytometry plots of Vδ2 γδ T-cell clusters, categorized into CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells. e Representative flow cytometry histograms and cumulative data presenting the PLZF expression in the Vδ2 γδ T-cell population ( n = 23 biologically independent donors). Violin plots showing expressions of MAIT gene set module score ( f ) and KLRB1 ( g ) in Vγ9Vδ2 clusters (C1–C3). Horizontal lines indicate the median expression level. h Proportions of IFN-γ + and TNF + cells among CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells after IL-12 and IL-18 stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). i Expression of IL-18Rα (CD218a) in the Vδ2 γδ T-cell population ( n = 12 biologically independent donors). j Representative flow cytometry plots and proportion of IL-17A + cells among CD26 + CD161 + (C1), non-CD26 + CD161 + (C2/C3), and MAIT cells as a positive control from peripheral blood (squares) and liver sinusoidal (circles) mononuclear cells after PMA/ionomycin stimulation (10 ng/mL; 500 ng/mL, 6 h) ( n = 8 biologically independent donors). Data are presented as mean ± s.d. Statistics: Gene set enrichment analysis with false discovery rate correction ( a ); two-sided Wilcoxon signed-rank tests ( b , e , h , i , j ); Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Single-cell landscape of human liver sinusoidal γδ T cells in the context of latent human cytomegalovirus infection

    doi: 10.1038/s41467-026-77255-z

    Figure Lengend Snippet: a Gene set enrichment analysis of Vγ9Vδ2 γδ T cells compared with non-Vγ9Vδ2 γδ T cells using published MAIT cell signatures ( GSE200173 ). NES, normalized enrichment score. b Proportion of IFN-γ + cells among non-Vδ2 and Vδ2 γδ T cells after IL-12 and IL-18 stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). c Violin plots showing ADT expression levels of CD26 and CD161 across Vγ9Vδ2 γδ T cell clusters (C1 and C2/C3). Horizontal lines indicate the median expression level. d Representative flow cytometry plots of Vδ2 γδ T-cell clusters, categorized into CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells. e Representative flow cytometry histograms and cumulative data presenting the PLZF expression in the Vδ2 γδ T-cell population ( n = 23 biologically independent donors). Violin plots showing expressions of MAIT gene set module score ( f ) and KLRB1 ( g ) in Vγ9Vδ2 clusters (C1–C3). Horizontal lines indicate the median expression level. h Proportions of IFN-γ + and TNF + cells among CD26 + CD161 + (C1) and non-CD26 + CD161 + (C2/C3) cells after IL-12 and IL-18 stimulation (50 ng/mL each, 18 h), with PMA/ionomycin as a positive control ( n = 12 biologically independent donors). i Expression of IL-18Rα (CD218a) in the Vδ2 γδ T-cell population ( n = 12 biologically independent donors). j Representative flow cytometry plots and proportion of IL-17A + cells among CD26 + CD161 + (C1), non-CD26 + CD161 + (C2/C3), and MAIT cells as a positive control from peripheral blood (squares) and liver sinusoidal (circles) mononuclear cells after PMA/ionomycin stimulation (10 ng/mL; 500 ng/mL, 6 h) ( n = 8 biologically independent donors). Data are presented as mean ± s.d. Statistics: Gene set enrichment analysis with false discovery rate correction ( a ); two-sided Wilcoxon signed-rank tests ( b , e , h , i , j ); Source data are provided as a file.

    Article Snippet: Cultures were incubated at 37 °C for 6 h. After the first hour of stimulation, brefeldin A (cat #555029; BD Biosciences) and monensin (cat #554724; BD Biosciences) were added, and their presence was maintained for the remaining 5 h. For intracellular cytokine staining (ICS) following IL-12 and IL-18 stimulation, cells were treated with recombinant human IL-12 (50 ng/mL; cat #200-12; PeproTech) and IL-18 (50 ng/mL; cat #9124-IL; R&D Systems) at 37 °C for 18 h. Brefeldin A and monensin were added during the final 5 h of incubation.

    Techniques: Analysis, Positive Control, Expressing, Flow Cytometry